- ￥2176
- 赛默飞（Thermo Fisher）
- 53-9743-80
- 2025年07月01日
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100
- 规格：
25 µg
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文献和实验Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
iodide at 50 µ g/ml in PBS with 100 U/ml RNase. Analyze on any 488 nm argon- 630 nm HeNe or diode dual laser flow cytometer for PI and Cy5 fluorescence. Both PI and Cy5 should be analyzed in linear mode. This technique has been used successfully
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
PBS/azide. Resuspend the cells in propidium iodide at 50 µg/ml in PBS with 100 U/ml RNase. Analyze on any 488 nm argon- 630 nm HeNe or diode dual laser flow cytometer for FITC, PI and Cy5 fluorescence. Both PI and Cy5 should be analyzed in linear
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry
5-conjugated anti-mouse IgG (available from Jackson ImmunoResearch and Caltag) in a volume of 200 µ l. Incubate for 4 hours at 4°C Wash once with cold cyclin staining buffer and once with cold PBS/azide. Resuspend the cells in propidium iodide at 50 µ g/ml
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