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文献和实验ELISA (Enzyme-Linked ImmunoadSorbent Assa
is from colourless to blue (yellow when acid is added). 2) Coat plate with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C. 3) Remove antigen and wash 3x with 1x PBS. 4) Block with 1% BSA (100
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
is from colourless to blue (yellow when acid is added). 2) Coat plate with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C. 3) Remove antigen and wash 3x with 1x PBS. 4) Block with 1% BSA (100
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