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文献和实验(Bio-craft). Fill the electrode tanks with 1x TBE buffer (3 L for the bottom tank). H. Running 2-D gel electrophoresis Rinse the top of the 2-D gel with 1x TBE and wipe with Kimwipe after in-situ digestion with MboI expel the gel noodle from tubing
Construction of BAC Libraries:Megabase DNA Isolation
with the same pipette tip, 100 ul per plug. When the agarose is completely solidified, transfer the plugs into 5-10 volumes of lysis buffer. 1. Before use, wash the agarose microbeads or the plugs containing HMW DNAs
MINICHROMOSOME MICROTUBULE BINDING ASSAY
Assault buffer, 0.5mL tRNA/øX174] tRNA/øX174 DNA: 1mL 1mg/mL tRNA in H2 O 0.1mL 10 ug/mL uncut øX174 DNA 5X BRB80 100mLs 5X stock 80mM Pipes pH6.8(KOH) 10mL 800mM Pipes pH6.8 (KOH) 1mM EGTA 1ml 100
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