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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 规格:
MCKLH- 10 MG.
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文献和实验make an Antibody against a Peptides
because the molluscs are harvested and maintained in salt water tanks. The characteristic blue, opalescent color of mcKLH is highly soluble, making coupling reactions easier to complete. Figure 1. Peptide Carrier Conjugation using Maleimide Activated
Phycoerythrin conjugation protocol
(Molecular Probes) DTT Dithiothreitol NEM N-ethyl maleimide * 10 ml Sephadex G-10 columns (2) Separation column A long Sephacryl S-300 column
Conjugation of Antibody to HRP
Antibody Concentrate the antibody to 5-7 mg/ml Dissolve 10 mg of SATA in 1 ml DMF (SATA:DMF) Add 3.0 ml SATA:DMF per mg of antibody Gently stir solution for 2 hours at room temperature. Dissolve 0.5 g hydroxylamine hydrochloride in 10 ml PBS
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