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文献和实验Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
pH 7.2, 140 mM NaCl) containing 0.1% Tween 20 and 5% non-fat milk. The levels of phosphorylated (Ser465/467) and total Smad2, and expression of vimentin, a characteristic mesenchymal marker, were analyzed with specific primary antibodies (Cell
on tumors, leading to poorer outcomes for patients. Recent studies demonstrate how Notch signaling increases tumor cell proliferation, and provide evidence that active Notch signaling maintains the cancer stem cell pool, induces epithelial-mesenchymal
为 20-24 个核苷酸的小 RNA 片段,主要在转录后水平通过与目的 mRNA 配对结合,影响靶蛋白的表达。作为一种高度保守的小分子 RNA,miRNAs 参与多种生理和病理过程。现有研究发现,miRNAs 能够通过影响肿瘤干细胞生物特性 (cancer stem-cell biology)、血管生成、上皮细胞间质化 (epithelial-mesenchymal transition,EMT) 和间质细胞上皮化 (mesenchymal-epithelial transition,MET
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