- ￥7069
- 赛默飞（Thermo Fisher）
- 7431-MSM11-P1
- 2025年07月10日
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文献和实验Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
pH 7.2, 140 mM NaCl) containing 0.1% Tween 20 and 5% non-fat milk. The levels of phosphorylated (Ser465/467) and total Smad2, and expression of vimentin, a characteristic mesenchymal marker, were analyzed with specific primary antibodies (Cell
Intracellular Cytokine Staining Protocol
TimeRestimulationIntracellular BlockAntibodyIL-1aNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinALF-161IL-1bNEW!mouse PECmINFg (100ng/ml)(2h4)/LPS (100ng/ml)(22hr)2hr/22hr-MonensinB122IL-2mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d
on tumors, leading to poorer outcomes for patients. Recent studies demonstrate how Notch signaling increases tumor cell proliferation, and provide evidence that active Notch signaling maintains the cancer stem cell pool, induces epithelial-mesenchymal
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