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文献和实验Microarray Approaches for Analysis of Tumor Suppressor Gene Function
TP53 Tumor protein p53 WT1 Wilm’s tumor 1 VHL Von Hippel-Lindau syndrome
AFLP: not only for fingerprinting, but for positional cloning
acid from mini(micro)prep) 5 U EcoRI 5 U MseI 8.0 µL 5x-Pharmacia "One-Phor-All+" buffer [10x = 100 mM Tris-acetate, pH 7.5, 100 mM Mg-acetate, 500 mM K-acetate) w/ 250 ng/µg BSA (=5x OPA+- BSA) Q.S. to 40 µL w/ dH20 - incubate @ 37° C for ~ 3 hrs
Protocol for mRNA amplification--RT-PCR实验过程
Blood Extraction kit Cat# 762134) ( see manufacturer's protocol). Resuspend total RNA in DEPC water at 1ug/ul concentration. Speedvac RNA down to a total of 3-4ug in 9uL DEPC H20. Prepare RNA in PCR tubes and store at �80C overnight
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