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100 µg
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文献和实验for large-scale purification (>25 µg) of poly(A)+ RNAextracted from mammalian cells. Typically, between 1% and 10% of the RNA applied to the oligo(dT) column is recovered as poly(A)+ RNA. Because the method can be frustratingly slow, it is not recommended
Overview of Formation of G‐Quadruplex Structures
., Bialis, T., Jones, R.A., and Yang, D. 2006. Human telomeric sequence forms a hybrid‐type intramolecular G‐quadruplex structure with mixed parallel/antiparallel strands in potassium solution. Nucleic Acids Res. 34: 2723‐2735
Native Chromatin Preparation and Illumina/Solexa Library Construction
off the PBS. Add 100 µL of 1X PBS to each tube. Add 4 µg of antibody to one tube and 4 µg of preimmune serum to the other. Rotate for 4-6 h at 4°C. 31. Using the magnetic stand, aspirate the supernatants and wash the beads in each tube
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