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文献和实验Adrenocorticotrophic Hormone and Related Peptides
This chapter describes how adrenocorticotropin (ACTH) can be measured in biological fluids. The principle can be applied to similar peptide hormones. Following an introduction of the principle of the method, details are given on sample
In Situ Hybridization Using Digoxigenin Labeled Probes
Volume 1) Mix DNA (100-500 ng) + ddH2O and boil 5 minutes. 2) Cool immediately on ice (only critical for small probes, cosmids and P1's seem to do fine at room temp.) 3) Add remaining reagents 4) Incubate at room temp. for several hours to overnight
1溶液配制: 1.1 Buffer P1:(悬浮用) 成分:50mM Tris.HCl, pH8.0, 10mM EDTA, 100ug/mL Rnase A 配制: 2M Tris.HCl, pH 8.0 25mL 0.5M EDTA, pH 8.0 20mL 双蒸水至 1L,灭菌。 使用前每1L buffer加入100mg RNAse A,并于4℃保存。 1.2 Buffer P2:(裂解用) 成分:200mM NaOH, 1% SDS 配制
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