- ￥6735
- 赛默飞（Thermo Fisher）
- 54-MSM3-P1
- 2025年06月30日
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100 UG
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
Volume 1) Mix DNA (100-500 ng) + ddH2O and boil 5 minutes. 2) Cool immediately on ice (only critical for small probes, cosmids and P1's seem to do fine at room temp.) 3) Add remaining reagents 4) Incubate at room temp. for several hours to overnight
carrying mTn3 derivative mTn-3xHA/GFP (GFP, URA3, tet ; tetracycline resistant) #70/B425 Strain NG135 (K12 recA56 gal-delS165 strA ; streptomycin resistant) with plasmid pNG54 (pACYC184 with mTn3 res and tnpR seqs; active
) with plasmid pNG54 (pACYC184 with mTn3 res and tnpR seqs; active resolvase, chloramphenicol resistant)(N. Grindley) B227 Strain DH5-alpha carrying pB227/GAL-cre (amp, ori, CEN, LEU2 ) (B. Sauer) Vector used
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