- ￥3479
- 赛默飞（Thermo Fisher）
- 50943-RBM5-P0
- 2025年07月16日
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文献和实验STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN
STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN Indirect Method Materials 1. Gc-globulin (vitamin-D binding protein; Calbiochem 345802), prepare 2 mg/ml stock in PBS solution A, clarify 25,000 rpm for 20 min in a 42
Intracellular Immunofluorescent Staining for Flow Cytometry
, it is necessary to block secretion of cytokines with protein transport inhibitors, such as Monensin or Brefeldin A , during the last few hours of the stimulation. It is advised that the investigators evaluate the use and efficacy of different protein transport
Site-directed Mutagenesis using PCR
mM Tris HCl, pH 7.5; 8 mM MgCl2; 40 ug/ml BSA); 12-20 pmole of each primer (one of which must contain a 5-prime phosphate), 250 uM each dNTP, 2.5 U Taq DNA polymerase, 2.5 U of Taq Extender (Stratagene).The PCR cycling parameters are 1 cycle of: 4 min
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