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文献和实验Single and Double FISH protocols for Drosophila
of IgG (H+L) 1/2000 dilution of a 1 mg/ml stock solution; Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA; Cat No. 115-226-062]. 7. Donkey anti-sheep antibody conjugated to CY3 [F(ab')2 fragment of IgG (H+L); 1/2000 dilution of a 1mg/ml
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
Mounting media: 70% glycerol, 1mg/ml p-phenylenediamine (pH 9). C. Working solutions These solutions are prepared on the day of use.- Formaldehyde fixative solution: 1X PBS, 0.08 M Hepes (pH 6.9), 1.6 mM MgSO4, 0.8 mM EGTA, 3.7% formaldehyde.-
Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells
dish (can store for a year). Coat with polyamino acid. Coat coverslips in bulk in 10-15ml 1mg/ml PLL (or 500ug/ml polyornithine), rocking or rotating for a minimum of 30 minutes in a 10 or 15cm tissue culture dish. Save the polyamino acid
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