- ￥4935
- 赛默飞（Thermo Fisher）
- 44975G
- 2025年07月08日
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文献和实验HIGH RESOLUTION GENETIC FOOTPRINTING
Trizma 72 g Taurine 4 g disodium EDTA (2 H2O) qs to 1 liter 1) LABEL GENE-SPECIFIC PRIMER WITH 32P 10 ul 10X T4 Polynucleotide Kinase buffer (New England Biolabs) 1 ul gene-specific primer (100 pmol) 10 ul (100 uCi) gamma-[32P]-ATP (3000 Ci
【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
nonspecific priming, and ensuring thatthe Tm of the primers is a few degrees higher than the intendedannealing temperature during thermal cycling but that both arein the same orientation as each other with the 3? ends pointingtowards the unknown region
in larger total volumes (between 50-100 ul), as should greater amounts of DNA. Refer to the vendor's catalog for the chart of enzyme activity in a range of salt concentrations to choose the appropriate assay buffer (10X High, 10X Medium, or 10X Low
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