- ￥7069
- 赛默飞（Thermo Fisher）
- 4176-MSM5X-P1
- 2025年07月13日
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文献和实验Array CGH of Microdissected Fresh or Formalin Fixed DNA
slide wet, then add 100 ml of RT 0.3ug/ml DAPI in PBS buffered glycerol, and carefully place a 22x50 mm coverslip over arrays, avoiding bubbles. Blot out excess DAPI and PN from the edges onto kimwipes and carefully seal the edges with clear nail enamel
Differential Display of Cotton Transcripts
, Madison, WI) 5X M-MuLV RT buffer (Promega, Madison, WI) 20 uM decamer primers (random) 20 uM anchored oligo-dT12MN primers (M=A,C, or G; N=A,C,G, or T) mixed dNTPs (2.5 mM each dNTP, 100 uM each dNTP Taq DNA Polymerase
Protocol cDNA-Microarray Hybridization
Volume: 19 µl (per tube) incubate for 3-5 min at 65 °C place on ice Make fluorescent cDNA probe 8 µl 5x first strand buffer 4 µl 0.1m DTT 4 µl 10x low dT dNTP 4 µl Cy
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