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100
- 规格:
200 µL
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文献和实验The major antigens secreted by the parasite Fasciola hepatica are cathepsin-L cysteine proteases. These enzymes can be isolated from the parasite excretory-secretory products in sufficient quantities for use as an antigen
Dual- and Triple-Color Fluorospot
100 μl/well of the mixed mAb solution and incubate overnight at 4–8°C (seeNote 1). c. After coating, empty the plate and wash five times with sterile PBS (200 μl/well) to remove excess mAb. Block the plate for at least 30 min with 200 μl
Immunofluorescent Staining of Mouse and Rat Leukocytes
cells from murine peripheral blood or a spleen cell suspension can be lysed using BD Biosciences Pharmingen's PharM Lyse™ (Cat. No. 35221E) solution. Add 2.0 ml of 1X Lysing Solution to the spleen cell suspension or per 200 µl of murine peripheral blood
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