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文献和实验Human Placental Alkaline Phosphatase Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike other AP proteins typically expressed by cells. A few cell types (HeLa cells, for example
Staining protocols for placental explant cultures
the fix and rinse in 0.1 M sodium cacodylate buffer pH 7.3. Dehydrate in an ascending alcohol series: 15 min each in 50, 70, 95, 100, 100% ethanol. Incubate in propylene oxide 2 x 15 min, in a fume cupboard, wearing protective gloves. Infiltrate
Correlative Microscopy of Ultrathin Cryosections in Placental Research
. Additionally, IEM can provide the “reference space” lacking in IFM. The use of ultrathin cryosections is a powerful approach for placental research, especially for the investigation of the in situ localization of antigens in the complex structure of the human
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