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文献和实验Molecular Measurement of T Cell Receptor Excision Circles
episomal circles of DNA are generated by the recombination process used to produce antigen-specific T cell receptors. The number of sjTRECs per mg of thymus tissue or per 100,000 lysed cells has been shown to be a molecular marker of thymopoiesis and na�ve
T-Cell Receptor Molecular Diagnosis of T-Cell Lymphoma
, and there is no reliable protein marker of malignancy. At the genomic level T-cell neoplasms clonally rearrange T-cell receptor (TCR) genes that can serve as clonal markers. In comparison with Southern blot analysis, polymerase chain reaction (PCR) techniques increasingly
Clonality - X Chromosome Inactivation Assay
) Forward and reverse primers, each 20 µM (Human Androgen Receptor Gene (HUMARA) CAG trinucleotide repeat microsatellite primers are described in Allen et al) AmpliTaq Gold, 5 U/ml (Perkin Elmer) a 32P-dCTP, 20 µCi/µl (NEN) Gel-Mix 6 (Life Technologies
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