- ￥4586
- 赛默飞（Thermo Fisher）
- 364000
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 规格：
100 UG
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文献和实验mm dish. This is about 100 ug protein for PC12 cells. After electrophoresis, electrotransfer to nitrocellulose membrane. I include in the SDS-PAGE prestained molecular weight markers to check the efficiency of the transfer
antibody: Anti-mouse IgG-Alkaline Phospphatase Buffers Lysis buffer: 100ml 50 mM Tris-Cl, pH 8.0 0.6057g 150m M NaCl 0.8766g 0.02%NaN3 0.02g 100ug/mlPMSF 0.01g 10ug/ml Aprotinin 0.001g 1% Triton X100 1g 2x SDS-loading buffer: 100ml 100 mM Tris
Intracellular Cytokine Staining Protocol
28 (2ug/ml soluble) 5hrMonensinJES5-16E3IL-12mouse PECmIFNg (100ng/ml) (2hr)/LPS (100ng/ml) (22hr)2hr/22hr-MonensinC17.8GM-CSFmouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble
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