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文献和实验Myosin Light Chain Preparation
1 and 2 with the Coomassie Blue Assay and B.S.A.standards.Run 10% SDS gels. 25.Do 32P assay to determine maximal extent of phosphorylation of light chain. 26.Scan gels with spectrophotometer at A 600.Cut out and weigh the peaks to determine % impurities and percent
Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Structure and Properties of Human Immunoglobulin Light-Chain Dimers
in that they are highly amenable to analysis by a fundamental scientific research strategy, i.e., investigation of complex systems by study of its parts. The metabolism of even a superficially simple bacterial cell cannot be understood without a rigorous understanding
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