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文献和实验Detection of Immunoglobulin Gene Rearrangements
heavy-chain (HC) genes, but correctly rearranged Ig light-chain (LC) genes. By contrast, latently infected B cells isolated from human peripheral blood have a memory B-cell phenotype, expressing both IgM and IgD ( 7 ). This chapter details
Immunoglobulin Class Switching: In Vitro Induction and Analysis
During an immune response, B lymphocytes can switch expression of immunoglobulin (Ig) class (isotype) from IgM to IgG, IgE, or IgA. This Ig class switch is based on a deoxyribonucleic acid (DNA) recombination event that results in an exchange
RNA Purification Protocol for 10-20mg of Animal Tissue
设备 1. Microcentrifuge capable of 13,000 x g 2. Sterile 1.5 mL microcentrifuge tubes 3. Ice bath 4. Microfuge tube pestle 实验步骤 1. Dissect tissue quickly and freeze in liquid nitrogen. Storage at -70°C
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