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文献和实验Gel Mobility Shift Assay for transcription factor binding
Gel Mobility Shift Assay for transcription factor binding Author: Hueley Lab Source: Contributed by Riddhish Shah, Hueley Lab Abstract: Protocol can be used
Reverse transcription is the reverse transcriptase-mediated synthesis of single-stranded DNA (complementary DNA or cDNA) using single-stranded RNA as template; the cDNA product and the template RNA have complementary sequence and can exist
RNA Purification Protocol for 10-20mg of Animal Tissue
. Fresh tissue can also be used. Work quickly and keep the sample on ice at all time. 2. Add 10-20 mg frozen ground tissue or fresh tissue to a 1.5 mL microtube containing 600ul RCL Buffer. Homogenize throughly using a microfuge tube pestle (Product
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