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- 赛默飞（Thermo Fisher）
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- 2025年07月10日
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文献和实验RUVKUN Antibody Staining Protocol revised 10/29/90
RUVKUN Antibody Staining Protocol revised 10/29/90 Fixation: 1. C. elegans N2 grown in liquid or plates. Harvest worms. Wash for 2 hours at room temp. in 1X M9 or PBS. 2. Fix in 5 ml of FRESH THAT DAY 1% paraformaldehyde:
Proteasome Preparation from Human Brain
Gamma Globulin H2 O 5mg 5ul 45ul 10mg 10ul 40ul 20mg 20ul 30ul 30mg 30ul 20
STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN
.2Ti rotor. 2. PBS/BSA: PBS solution A with 1% BSA (Boehringer Mannheim 100 350) and 0.1% NaN3 , stored at 4o C. 3. Diluted Gc-globulin, dilute 2 mg/ml stock with PBS/BSA to obtain 1-10 ug/ml. Need 200 ul per coverslip. 4. Primary
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