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文献和实验Stool DNA Protocol (for pathogen detection)
the sample on ice for 2 minutes. 5. Add 100 μl of Buffer SP2. Mix the sample throughly by voretxing the tube for 30 seconds. 6. Incubate the sample on ice for 5 minutes. 7. Centrifuge at full speed (13,000-20,000 x g) in a microcentrifuge
RNA Purification Protocol for 10-20mg of Animal Tissue
设备 1. Microcentrifuge capable of 13,000 x g 2. Sterile 1.5 mL microcentrifuge tubes 3. Ice bath 4. Microfuge tube pestle 实验步骤 1. Dissect tissue quickly and freeze in liquid nitrogen. Storage at -70°C
Intracellular Cytokine Staining Protocol
spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD28 (2ug/ml soluble) 5hrMonensinMP5-20F3IL-10mouse spleenConA (3ug/ml) (2d)/IL-2 (20ng/ml)+IL-4 (20ng/ml) (3d)2d/3danti-CD3 (10ug/ml immobilized)+anti-CD
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