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文献和实验RNA Purification Protocol for 10-20mg of Animal Tissue
设备 1. Microcentrifuge capable of 13,000 x g 2. Sterile 1.5 mL microcentrifuge tubes 3. Ice bath 4. Microfuge tube pestle 实验步骤 1. Dissect tissue quickly and freeze in liquid nitrogen. Storage at -70°C
Plant DNA methylation analysis by bisulfite genomic sequencing
(12000 X g); Discard supernatant, vacuum dry for 10 minutes at room temperature; Dissolve cut DNA in 10µl bidist water (0.25 or 2.5 µg/µl, respectively), store at -20°C; Bisulfite treatment The bisulfite solution
Whole genome amplification protocol for ChIP-chip
of different factors (E2F family members, KAP1, CtBP2, ZNF217) as well as on histone modifications (H3me3K9, H3me3K27, H3me3K4) (Krig et al., 2007; O'Geen et al., 2007). Another benefit of the WGA amplification method is the ability to perform a second
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