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文献和实验the concentration of unbound FA is a method using the fluorescent-(acrylodan) labeled intestinal FA-binding protein (ADIFAB). Under appropriate conditions, ADIFAB fluorescence measures unbound FA, regardless of any third phase such as albumin, FA-binding proteins
Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes
The authors’ laboratory has applied a series of different fluorescence assays for monitoring the binding and transport of fatty acids (FA) in model and biological membranes. The authors recently expanded their fluorescent assays
A Scintillation Proximity Assay for Fatty Acid Amide Hydrolase Compatible with Inhibitor Screening
that bind to human fatty acid amide hydrolase (FAAH). This SPA assay utilizes the specific binding of [3 H]-R (+)-methanandamide (3 H-MAEA), a competitive nonhydrolyzed FAAH inhibitor, to FAAH expressing microsomes and evaluates its displacement by FAAH
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