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100
- 规格:
100 uG
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文献和实验Cytochrome Oxidase Manometric Analysis
inversion. Add 100 µ l of PPDO to the tube and immediately measure its absorbance at 630 nm. Continue to measure the absorbance every 30 seconds for 4-5 minutes or until there is no further change in absorbance. Note that oxidation
Methods for the Detection of D-Amino-Acid Oxidase
Abstract Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described. Introduction
to 0.05, and zymolyase (100T) to 60 ug/ml. Incubate 1 hour at 37oC. Pellet spheroplasts as above. Resuspend in 10 mls Solution A and pellet again. Combine the suspension of whole cells with the spheroplasts and pellet as above. Resuspend in 10
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