- ￥25799
- 赛默飞（Thermo Fisher）
- 16-4714-38
- 2025年07月07日
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5 MG
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文献和实验). Purified embryos were resuspended in 100 l of M9 to which 100 l of 1 mg/ml chitinase (Sigma, St. Louis, MO) dissolved in 0.3M mannitol, 50mM HEPES (pH 7.2), 10mM NaCl, 10mM MgCl2 , 2mM DTT was added and shaken vigorously in a microcentrifuge tube for 70
Methyl DNA Immunoprecipitation
. The MBD method combined with enzymatic restriction of the DNA has been adapted to HTP (8 , 12 ). Yet another way to enrich for methylated DNA is by Methyl DNA IP. Methyl DNA IP uses bead-immobilized anti-5-methyl cytosine antibodies to isolate
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
of a reporter gene also requires consideration. In order to see all the progeny of infected cells, a constitutive promoter should be used. We have had success using the LTR of Moloney Murine Leukemia Virus (Mo-MLV) for work in rats and mice and the LTR of Avian
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