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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
B. TdT buffer 30 mM Trizma base (pH 7.2), 140 mM sodium cacodylate, 1 mM cobalt chloride 4°C B. TB buffer 300 mM sodium chloride, 30 mM sodium citrate RT B
定量RT-PCR (Quantitative RT-PCR)
(8.5 ml per well =1 mg per well).b. Add 1 ml of the diluted control RNA in each well in the order of 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, 10 fg, 1 fg. c. Add 10.5 ml reverse transcription mixture to each well. RT mixture: 3' antisense primer
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
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