- ￥6828
- 赛默飞（Thermo Fisher）
- 112939-MSM2-P1ABX
- 2025年07月14日
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- 文献和实验
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- 库存：
100
- 规格：
100 UG
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文献和实验or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
CaPO4 Transfection for Chromaffin Cells
with 1.0 M NaOH. Adjust volume to 100 ml. If desired, filter sterilize. Store at 4'C N.B.: The above amounts assume the free acid ot PIPES. For NaPIPES use 1.30g. 4. DNA in TE of H2O 115 ug/ml (this is the optimum amount). Procedure:
CaPO4 Transfection for Chromaffin Ce
with 1.0 M NaOH. Adjust volume to 100 ml. If desired, filter sterilize. Store at 4'C N.B.: The above amounts assume the free acid ot PIPES. For NaPIPES use 1.30g. 4. DNA in TE of H2O 115 ug/ml (this is the optimum amount). Procedure:
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