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文献和实验Making antibodies to synthetic peptides
, from which the peptide was derived), especially on immunoblot. Sometimes the epitope is weak, and the antobodies are of low titer (e.g., 1:100 for immunoblot). But we always get a usable antibody. Citations where we've used these procedures: Barbosa JA, Gill BM
Making antibodies to synthetic pepti
, from which the peptide was derived), especially on immunoblot. Sometimes the epitope is weak, and the antobodies are of low titer (e.g., 1:100 for immunoblot). But we always get a usable antibody. Citations where we've used these procedures: Barbosa JA, Gill
Adrenal chromaffin granule (chromaffin vesicle) preparation
of 1.6 M sucrose. 10. Spin the gradients at 0-4 o C for 10 hours (or overnight) in a refrigerated centrifuge (we use a Sorvall and a fixed angle rotor) at 10 KG. This reproduces the number of (G)(min) achieved by a 100 KG spin for 1 hour
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