- ￥790
- 赛默飞（Thermo Fisher）
- 12-0110-80
- 2025年07月11日
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
of one rat spleen (approx. 10**8 cells) together with 6 x 10**7 Y3/Ag1.2.3 per fusion (the other half of the spleen cells can be frozen as a backup in 90% FCS +10% DMS0). c) Try and keep all cells, PEG and medium components at 37 deg. C
Immunofluorescent Staining of Mouse and Rat Leukocytes
(or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g
50T/100T 800/1200 L0031 Mouse anti-Human CD11a mAb,PE 50T/100T 1000/1500 L0032 Mouse anti-Human CD11b mAb,FITC 50T/100T 800/1200
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