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文献和实验Cytokines: (Peprotech) mu SCF hu IL-6 hu Flt-3L hu IL-11 Thrombopoeitin hu IL-3 Erythropoeitin (Amgen) PROCEDURE Harvest Marrow: 1. Flush femurs with PBS/FBS and pass cells through a 23G needle
Single-molecule analysis of DNA replication by molecular combing
The protocol described below is meant to detect BrdU incorporation in yeast cells arrested in early S phase with hydroxyurea (HU). It can be easily adapted to monitor ongoing DNA replication in asynchronous yeast cultures or to analyze DNA replication
secondary antibody review -- data from 99 publications
epithelial Caco2/bbe cells Bio-Rad 10 rhodamine immunocytochemistry 1:100 detect phospho-CREB in human colonic epithelial Caco2/bbe cells Bio-Rad 11 goat IgG FITC immunohistochemistry 1:40 detect ameloblastin in mouse postnatal mandibular molars
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