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文献和实验buffer; 1ml of ChIP high salt buffer; 1ml of ChIP wash buffer; (note 4)Pulldown (2), Flag-tag specific After the last wash elute the complex by adding 100µl of ChIP elution buffer. Incubate the samples at 65°C for 10 minutes
TSA Treatment of Mammalian Cells (PROT09)
enabling progression through multiple cell divisions. We usually use the following range: 5, 15, 25, 50 and 100ng/ml diluted in culture media. Drug and medium are renewed every 2 days (TSA is unstable) . We follow the cell culture over a week
Chromosome Conformation Capture (3C) (PROT05)
Introduction An alternative protocol (PROT31) for 3C has been adopted by the author's lab. Much of it is identical to this version. The major differences are in the amounts of DNA used at different steps. We reliably
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