- ￥16690
- 赛默飞（Thermo Fisher）
- 100-601-206
- 2025年07月09日
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文献和实验Alanine Transaminase Activity Assay
2) Alanine Transaminase Substrate: Reconstitute with 1.1 ml Assay Buffer. Aliquot and store at -20oC. Use within two months. 3) Alanine Transaminase Positive Control: Reconstitute with 100 µl dH2O. Aliquot and store at -20oC, use
the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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