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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
咨询甄准
- 保质期：
咨询甄准
- 英文名：
4,4'-Isopropylidenebis[2-(2,6-dibromophenoxy)ethanol]
- 库存：
1
- 供应商：
上海甄准
- CAS号：
4162-45-2
- 规格：
10g
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文献和实验is separated from the DNA by loading the solution onto an equilibrated ion exchange column. The A260 containing fractions are pooled, diluted, and ethanol precipitated, and the final DNA pellet is resuspended in buffer and assayed by restriction digestion
7.7 to a final concentration of 2.5 M and 2.5 volume of ice-cold 100% Ethanol to precipitate the DNA. 26. Centrifuge at 10,000 X g for 10 min at 4°C to pellet the DNA. 27. Wash the DNA pellet with 70% Ethanol. 28. Centrifuge at 10,000 X g for 10 min at 4°C
min at 4°C to pellet the DNA. 27. Wash the DNA pellet with 70% Ethanol. 28. Centrifuge at 10,000 X g for 10 min at 4°C to pellet the DNA. 29. Resuspend the DNA pellet in 50 μl of TE. Protocol Hints 1. The Phenol:Chloroform phase
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