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- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Sulfur from Na2SO4 100 ug/mL in H2O
- 库存：
1
- 供应商：
甄准生物
- 规格：
250ML
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文献和实验ELISA (Enzyme-Linked ImmunoadSorbent Assay)
(yellow when acid is added). 2) Coat plate with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C. 3) Remove antigen and wash 3x with 1x PBS. 4) Block with 1% BSA (100ul/well) in 1x PBS
(交流)ELISA (Enzyme-Linked ImmunoadSorbent Assay)
with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C.3) Remove antigen and wash 3x with 1x PBS.4) Block with 1% BSA (100ul/well) in 1x PBS for at least 1hr at 37°C.5) Remove BSA by flicking plate
of a 10% gel 4 ml H2O 3.3ml 30% acrylamide/bisacrylamide (29:1 mix) 2.5 ml1.5M Tris(PH 8.8) 10% SDS 100 µl 10% ammonium persulfate 100 µl TEMED 8 µl 2. Stacking gel: 4 ml 2.7ml water 1 ml 0.67ml 30% acrylamide/bisacrylamide (29:1 mix) 0.ml 1.0M
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