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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
peptide
- 亚型:
可咨询销售
- 形态:
液体
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles
- 克隆性:
Monoclonal Antibody
- 适应物种:
Human, Mouse, Rat
- 保质期:
12个月
- 抗原来源:
Mouse
- 级别:
一级
- 库存:
88
- 供应商:
澳睿赛生物技术(上海)有限公司
- 宿主:
Mouse
- 应用范围:
WB=1/1000-1/1000;IP=1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
- 浓度:
可咨询销售
- 抗体英文名:
4E-BP1/2/3 (Phospho-Thr 45)
- 抗体名:
4E-BP1/2/3 (Phospho-Thr 45)
- 规格:
100μl/50μl
| 规格: | 100μl | 产品价格: | ¥2180.0 |
|---|---|---|---|
| 规格: | 50μl | 产品价格: | ¥1280.0 |
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
monoclonal antibody production fusion
), 3 L, 0.22 (or 0.45) µm-filtered, store at -20ѓC = Asp-Arg x100, 1 L + Gluta x 50, 2 L + mercaptoethanol, 350 µl Asp-Arg x100, 1 L, pH 6.0 = L-Asparagine, 3.6 g (0.24 mM) + L-Arginine (0.55 mM), 11.6 g + HCl, 0.5N, 100 ml -> heat to 37ѓC
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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