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- 规格:
100ul/500ul
| 规格: | 100ul | 产品价格: | ¥450.0 |
|---|---|---|---|
| 规格: | 500ul | 产品价格: | ¥1620.0 |
Product Type
Physical State
Antibody Source
Secondary Antibody
Liquid
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled
to agarose beads
Antibody Purity
Suggested Dilution
Range
Fluorophore
Fluorophore/Protein
Ratio
Storage Buffer
Stabilizer
Preservative
Storage Instructions
Note
The antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other
rabbit immunoglobulins. Cross-reactivity with bovine, chicken, goat, guinea pig, hamster, horse,
human, mouse, rat and sheep IgGs has been minimized with affinity procedures.
1:100- 1:800 for most applications
DyLight 594-TFP ester
Amax=591 nm; Emax=616 nm
Moles DyLight 594 per Mole Antibody
0.01M Sodium phosphate, 0.15M NaCl, 50% Glycerol, pH7.2.
10 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
0.05% Sodium Azide
Store at-20°C. Avoid repeated freeze / thaw cycles.
The product listed herein is for research use only and is not intended for use in human or clinical
diagnosis. Nothing disclosed herein is to be construed as a recommendation to use this product in
violation of any patents. The information presented above is believed to be accurate. However, said
information and product are offered without warranty or guarantee since the ultimate conditions of
use and the variability of the materials treated are beyond our control. We cannot be responsible for
patent infringements or other violations that may occur with the use of this product

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文献和实验人抗风疹病毒IgG抗体(anti-RV IgG)酶联免疫分析(ELISA)
中依次加入抗风疹病毒 IgG 抗体( anti-RV IgG ),再与 HRP 标记的抗原结合,形成抗原 - 抗体 - 酶标抗原复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗风疹病毒 IgG 抗体( anti-RV IgG )呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中人抗风疹病毒 IgG 抗体( anti-RV IgG )浓度。 试剂盒组成
,但是Dylight的效价通常是常规荧光二抗的2-4倍左右,因此就最终的性价比,Dylight更胜一筹。举例来说,EarthOx的FITC标记山羊抗小鼠IgG二抗 (货号为E031210 ),100μl和500μl报价分别是¥160和¥640;而相应的EarthOx的Dylight 488标记的山羊抗小鼠IgG二抗 (货号为E032210 ),100μl和500μl报价分别是¥200和¥800,但是在具体使用时,比如免疫荧光实验,其推荐稀释比率分别为1:100和1:400,即100μl和500μl的FITC标记山羊抗小鼠IgG
【共享】荧光二抗的选择——Dylight荧光与传统荧光标记的比较
比,Dylight更胜一筹。举例来说,EarthOx的FITC标记的山羊抗小鼠IgG二抗(货号为E031210),100μl和500μl报价分别是¥160和¥640;而相应的EarthOx的Dylight 488标记的山羊抗小鼠IgG二抗(货号为E032210),100μl和500μl报价分别是¥200和¥800,但是在具体使用时,比如免疫荧光实验,其推荐稀释比率分别为1:100和1:400,即100μl和500μl的FITC标记山羊抗小鼠IgG二抗(货号为E031210)最终使用液是10ml和50
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