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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Mivacurium Chloride (mixture of isomers)
- 库存：
1
- 供应商：
甄准生物
- CAS号：
106861-44-3
- 保存条件：
Hygroscopic, -20°C Freezer, Under Inert Atmosphere
- 规格：
2.5mg / 25mg
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文献和实验CALCIUM CHLORIDE TRANSFORMATION OF BACTERIA WITH PLASMIDS
it with a pipet tip, and incubate the mixture on ice for 20 minutes. Heat shock the mixture for 2 minutes at 42℃ (37℃ is also fine). Transfer the bacterial suspension to 10 ml of L Broth without antibiotic. Shake for 45 minutes in a 37℃ incubator. Centrifuge
Cesium Chloride Purification of T7
1 molar. Centrifuge the lysate at 10,000 rpm for 10 min, Discard the cellular debris, and add 10 grams polyethylene glycol (PEG) m.w. 8000 (10% w/v) to the supernatant. Gently stir the mixture until the PEG has totally dissolved. Keep lysate on ice
to insure that the correct sequence is represented. The melting temperature in buffered saline solution of this mixture of oligonucleotides is heterogeneous due to differences in G + C content, as G:C base pairs possessing three hydrogen bonds interact
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