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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, aliquot and store at -20°C.
- 保质期:
参照说明书可保存1-2年
- 英文名:
BAM 22P Peptide
- 库存:
一定量库存
- 供应商:
华拓生物
- 规格:
1 mg
| 产品名称: | BAM 22P Peptide |
| 产品链接: | http://www.abbiotec.com/peptides/bam-22p |
| 货号: | 350076 |
| 规格: | 1 mg |
| 产品描述: | BAM 22P is a 22-aa peptide corresponding to aa210-231 of the precursor proenkephalin-A. It encompasses the sequence of Met-enkephalin that mimics the effects of opiate drugs, which are pain perception and stress response. BAM 22P peptide is a potent endogenous agonist for the sensory neuron specific receptor (SNSR) and isolated from bovine adrenal medulla. EC50 values are 13 and 16 nM for human SNSR3 and SNSR4 respectively. BAM 22P produces opioid- and non-opioid receptor mediated antinociceptive effects in vivo. |
| 序列: | Human, Mouse, Bovine: H-Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu-Trp-Trp-Met-Asp-Tyr-Gln-Lys-Arg-Tyr-Gly-OH or H-YGGFMRRVGRPEWWMDYQKRYG-OH |
| 分子式: | C130H184N38O31S2 |
| 分子量: | 2839.28 g/mol |
| 纯度: | Purity > 95% by HPLC |
| 别名: | Bovine adrenal medulla docosapeptide; BAM 22P; Adrenorphin; Met-enkephalin; Opioid growth factor; OGF; PENK |
| 形态: | Each vial contains 1 mg of lyophilized solid packaged under an inert gas and supplied as a trifluoroacetate salt. |
| 溶解方法: | Distilled water for a solution up to 2 mg/ml, otherwise we recommend using acetonitrile. |
| 保存方法: | Store at -20°C. The product is hygroscopic and must be protected from light. Product is guaranteed one year from the date of shipment. Following reconstitution, aliquot and store at -20°C. |
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文献和实验22 (BAM-22) SNSR3 and SNSR4 Peptide library Neuropeptides B and W GPR7 and GPR
,其整个操作程序完全不同于AP-PCR和DD-PCR。前2种方法是将两模板DNA或cDNA分别进行PCR扩增,最后通过扩增产物的差异,分离和克隆特异扩增带,其缺点是需要将特异扩增产物从胶中切下来,提取DNA,再扩增后才能克隆。RDA-PCR只扩增区别于某一表型的特异基因,因而更便于扩增产物的克隆与分析(见图5)。其基本原理是:用一个在种源上相近的基因组将靶基因组中所有共同的基因掩盖起来,而只暴露出特异的基因,在整个反应中只有特异基因能被扩增。其操作程序为:(1)用同一限制性内切酶(一般用Bam HI
上相近的基因组将靶基因组中所有共同的基因掩盖起来,而只暴露出特异的基因,在整个反应中只有特异基因能被扩增。其操作程序为:(1)用同一限制性内切酶(一般用Bam HI,Bgl II或Hind III)同时处理靶基因组和掩盖基因组DNA,其中掩盖基因组DNA的量至少要2倍于靶基因组DNA的量;(2)在经酶切的靶基因组片段的两端加上连接头,其序列与酶切产生的粘性末端的序列相对应;(3)将2个基因组的DNA混合后,高温变性,低温退火。由于掩盖基因组DNA的量远大于靶基因组DNA量,那么与掩盖基因组DNA相同
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