2W LTII CHAMBERED COVERGLASS(M

Automated Three‐Chambered Social Approach Task for Mice

.    Crawley, J.N., Chen, T., Puri, A., Washburn, R., Sullivan, T.L., Hill, J.M., Young, N.B., Nadler, J.J., Moy, S.S., Young, L.J., Caldwell, H.K., and Young, W.S. 2007. Social approach behaviors in oxytocin

免疫荧光操作（共聚焦显微镜）-Immunofluorescence Protocol for Confocal Microscopy

of Molecular Probes Slowfade Light antifade medium. Pipet about 15 m l on slide and coverslip. For chambered coverglass just put two-three drops in each chamber after wash **This is a "light" fix due to the sensitivity of the antigen(s). Cells

Immunofluorescence / Confocal Microscopy Protocol

实验步骤   B or T cells in suspension, adherent cells on chambered coverglass or chamberslides, cryostat sections of unfixed, OCT embedded tissue: 1. Wash cells 1x cold RPMI (no wash for cryostat sections). 2. Fix 20