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- 技术资料
- 保存条件：
0 TO 5 C
- 供应商：
西宝生物
- 英文名：
ALBUMIN, BOVINEPATH 4 35% SOLUTION
- 库存：
50 ml
- 规格：
IMMUNOBIOLOGICALS
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文献和实验In vitroculture of early chick embryos
1. Use sterile technique. Prewarm Howard's Ringers in petri dish and agar/albumin culture dish to 37ºC. 2. Crack 2-day egg into large sterile petri dish. Alternately, open blunt end of egg
in a J6M rotor.6. Remove the supernatant and resuspend the pellet in 200 to 500 μl DNase solution and incubate at 37℃ for 15 min.7. Extract the reaction solution by adding an equal volume of SEVAG and vortexing vigorously. Centrifuge for 30 min at 35,000
the nucleic acids by centrifuging at 35,000 rpm at 4℃ for 20 min in a J6M rotor. 6. Remove the supernatant and resuspend the pellet in 200 to 500 μl DNase solution and incubate at 37℃ for 15 min. 7. Extract the reaction solution by adding an equal
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