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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
N-(6-Methoxy-1,3-benzothiazol-2-yl)guanidine
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
86328-45-2
- 规格:
10mg///100mg///200mg
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文献和实验: 2‐(N ‐(2‐(4‐((N ‐(2‐(Bis(4‐methoxyphenyl)(phenyl)methoxy)ethyl)‐2‐(uracil‐1‐)acetamido)methyl)‐1H‐1,2,3‐triazol‐1‐yl)ethyl)‐2‐(uracil‐1‐yl)acetami‐do)ethyl (2‐cyanoethyl) diisopropylphosphoramidite (24 ; Basic Protocol
PCR Genotyping of Embryos and Blastocysts
then diluting some of the sample in a standard PCR rxn mixture. The really interesting part about this paper is it describes a method for embryo biopsy in which a single blastomere is removed for PCR and the remaining embryo is returned, after incubating O/N
from frozen stock.Wash the cell pellets two times in ice cold PBS, and depending on size of pellet make up in 300 - 600µl of TE, pH 8.Add 7.5 volumes of 6 M guanidine HCl/0.1 M NaAcetate (17.2mg GuHCl and 1 ml of 3 M NaAcetate made up in 30ml of ddH2O).Mix
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