EGFR重组蛋白|Recombinant Human EGFR Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)

EGFR重组蛋白|Recombinant Human EGFR Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)（产品说明）
蛋白名称：Human EGFR Protein (Site-Specific AF 647 Conjugation, ECD, His Tag)A DNA sequence encoding the extracellular domain (Met 1-Ser 645) of human EGFR (NP_005219.2) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with AF 647 (Excitation Max.= 655 nm, Emission Max.= 680 nm).
表达宿主：HEK293 Cells
蛋白纯度：1.Flow cytometric analysis of anti-EGFR CAR expression. 5e5 of anti-EGFR CAR-293 cells were stained with 100 μL of 1 μg/ml of AF 647-conjugated human EGFR Protein (Site-Specific AF 647 Conjugation, ECD, His Tag, Cat. No. 10001-H86H-SG) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 647 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 647-conjugated EGFR protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and AF 647-conjugated human EGFR Protein (Site-Specific AF 647 Conjugation, ECD, His Tag, Cat. No. 10001-H86H-SG) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).< 1.0 EU per μg protein as determined by the LAL method.Leu 25The protein has a predicted molecular mass of 74.3 kDa.NP_005219.2Met1-Ser645