氘21-三异丙基磷硫化碳复合物 D21-Tri-iso-p

32P-postlabelling for the Sensitive Detection of DNA Adducts

. Prior to the development of the assay, quantification of DNA adducts was confined to studies that utilised compounds synthesised to be isotopically labelled with tritium or carbon-14. As such, these studies were limited to specific and recognised genotoxins

Site-directed hydroxy radical mapping of nucleosome positions in vitro

If the reaction is also supplied with a reducing agent (e.g. ascorbic acid or DTT), the iron III product can be recycled to iron II so that the iron/chelator complex acts as a catalytic centre (Figure 2). Generating multiple hydroxyl radicals per molecule

分享 生物化学名词解释完全版

性抑制作用（uncompetitive inhibition）: 抑制剂只与酶-底物复合物结合而不与游离的酶结合的一种酶促反应抑制作用。这种抑制使Km和υmax都变小但υmax/Km不变。 19，丝氨酸蛋白酶（serine protease）: 活性部位含有在催化期间起亲核作用的丝氨残基的蛋白质。 20，酶原（zymogen）:通过有限蛋白水解，能够由无活性变成具有催化活性的酶前体。 21，调节酶（regulatory enzyme）:位于一个或多个代谢途径内的一个关键部位的酶，它的活性根据代谢的需要而增加或降低。 22