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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
D6-Minocycline sulfate salt
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:1036070-10-6 free base;unlabelled:10118-90-8 free base
- 规格:
1mg///5mg///10mg
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文献和实验Site-directed hydroxy radical mapping of nucleosome positions in vitro
). Alternatively, fragments can be isolated by restriction digestion of plasmids after large scale plasmid preparations (see note 9); DNA fragments should be strand-specifically labelled, for example with a fluorophore dye or radioisotope (see note 10
In situ hybridization of mammalian cells (RNA and Oligonucleotide probes)
are rehydrated for 5 minutes at room temperature, in 2x SSC (300 mM NaCl, 30 mM sodium citrate, pH 7.0), 50% formamide. Cells are hybridized overnight at 37C in 40 µl of a mixture containing 10% dextran sulfate, 2 mM vanadyl-ribonucleoside complex, 0.02% RNAse
In situ hybridization of mammalian cells (RNA and Oligonucleotide probes)
sodium citrate, pH 7.0), 50% formamide. Cells are hybridized overnight at 37C in 40 µl of a mixture containing 10% dextran sulfate, 2 mM vanadyl-ribonucleoside complex, 0.02% RNAse-free BSA, 40 µg E.coli tRNA, 2x SSC, 50% formamide, 30 ng of probe
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