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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
1,2,3,4-13C4-Sodium Octanoate
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:1313734-79-0;unlabelled:1984-06-1
- 规格:
1mg///5mg///10mg
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文献和实验Generation of uni-directional nested deletions in plasmid DNA
for at least 2 minutes at the desired temperature. 6) Prepare 31 sterile eppendorf tubes, each containing 3μl S1 nuclease/S1 nuclease buffer (20μl 5x S1 nuclease buffer, 79μl H2O, 1μl S1 nuclease [50U/ul]), labelled as time (t)= 0-30 minutes. Place the tubes on ice
Generation of uni-directional nested deletions in plasmid DNA
, 79ul H2 O, 1ul S1 nuclease [50U/ul]), labelled as time (t)= 0-30 minutes. Place the tubes on ice. 7) Remove a 2ul aliquot of the pre-incubated deletion mix into the tube labelled t=0 and hold on ice. Return the deletion mix to the water bath
Generation of uni-directionalnesteddeletion sin plasmid DNA
sterile eppendorf tubes, each containing 3μl S1 nuclease/S1 nuclease buffer (20μl 5x S1 nuclease buffer, 79μl H2 O, 1μl S1 nuclease [50U/ul]), labelled as time (t)= 0-30 minutes. Place the tubes on ice. 7) Remove a 2μl aliquot
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