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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
D3-Hexanoyl-L-carnitine HCl
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:2483831-95-2;unlabelled:162067-53-0
- 规格:
1mg///5mg///10mg
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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
with ethidium bromide and irradiation with UV. The major bands correspond to 28S and 18S rRNA, faint bands to 23S, 16S and 5S rRNA. Figure 2 . Autoradiogram after Northern blotting and hybridization to 32P-labelled cDNA insert of arbitrarily chosen clone
Fast and reliable mini-prep RNA extraction from Neurospora crassa
. Visualization was done by staining with ethidium bromide and irradiation with UV. The major bands correspond to 28S and 18S rRNA, faint bands to 23S, 16S and 5S rRNA. Figure 2 . Autoradiogram after Northern blotting and hybridization to 32P-labelled cDNA
30min。 (7)缓冲液i 15min×2室温。 (8)缓冲液III(100mmol/l Tris -HCl, 100mmol/L NaCl, 50mmol/L MgCl 2 ,pH9.5)室温2min。 5.显色 (1)显色液配制:缓冲液III:1ml中加入4.5μl四氮唑蓝(NBT),3.5μl X-磷酸盐(5-溴-4-氯-3吲哚磷酸盐(BCIP配成))。30μl/每张切片,置暗处显色30min到2h。定时抽查切片
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