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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
13C,D3-Rabeprazole sodium salt
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:1261392-48-6 free base
- 规格:
1mg///5mg///10mg
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文献和实验Site-directed hydroxy radical mapping of nucleosome positions in vitro
by labelling with different dyes. Cy5 is preferred because of its better signal to noise ratio in gels. For radioactive labelling of PCR-prepared DNA fragments, we combine a primer for the strand to be labelled with a free 5' hydroxyl together with a primer
You will need: Freshly made 2M NaOH 3M sodium acetate, pH 4.5 Sterile, distilled water Absolute ethanol 70% ethanol 7 x DNA annealing buffer (280mM Tris.Cl, pH 7.5, 100mM MgCl2, 350mM NaCl) Termination mixes (40mM Tris.Cl, pH 7.5, 50mM NaCl, 10mM MgCl2, 150mM dTTP
) (store at 4deg.C). IPTG (isopropyl b-D-thiogalactopyranoside):25 mg/ml IPTG in double distilled water 250 mg IPTG (Sigma I-5502) ddH2 O to 10 ml (aliquot and store at -20℃) 1 M isocitrate (sodium salt-dihydrate) : 29.41 g Na3isocitrate-2H
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