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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
15N-Ammonium Acetate
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:86451-35-6;unlabelled:631-61-8
- 规格:
1mg///5mg///10mg
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文献和实验Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
protein-protein interactions with in vitro transcribed and translated proteins. The two proteins should be translated such that one is labelled with 35S methionine and one is unlabelled. If binding is to be assayed by Immunoprecipitation
is too few. Resuspend in nXml of TCM-N3 where n is the number of tubes you will have. Pipette 1 ml of the cell suspension into each of your 12x75 mm tubes (having labelled them first). Centrifuge for 5 minutes at 1500 rpm. Aspirate all but about 10 µl
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